Recombinant Anti-Topoisomerase II alpha Antibody [rTOP2A/6569] (A277925)

Anti-Topoisomerase II alpha Antibody [rTOP2A/6569]

Recombinant mouse monoclonal [rTOP2A/6569] antibody to Topoisomerase II alpha.

It recognizes a 170kDa protein, which is identified as topoisomerase IIα. It shows no cross-reaction with Topoisomerase II β or I. Topo II α plays important roles in synthesis and transcription of DNA as well as chromosomal segregation during mitosis. It is reported to be a sensitive and specific marker of late S-, G2- & M-phases in transformed and developmentally regulated normal cells. Topo II α is also implicated in drug resistance of tumor cells and has been shown to be over-expressed in many human cancers. Decreased expression of Topo IIa is the predominant mechanism of resistance to several chemotherapeutic agents.

WB, IHC-P

WB: 1-2 µg/ml, IHC-P: 0.5-1 µg/ml

Human, Mouse

Recombinant fragment, around amino acids 1,352-1,493, of human Topoisomerase II alpha. The exact sequence is proprietary.

Mouse

Monoclonal

rTOP2A/6569

IgG2a

kappa

Protein A/G chromatography.

200 µg/ml

170 kDa

Liquid

Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide.

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

This monoclonal antibody is also available in a different formulation without BSA and Sodium Azide - Anti-Topoisomerase II alpha Antibody [rTOP2A/6569] - BSA and Azide free (A278513).

DNA topoisomerase 2-alpha, DNA topoisomerase II, alpha isozyme, TOP2