Rabbit polyclonal antibody to Topoisomerase II alpha.
Applications
WB
Dilutions
WB: 1:500-1:1,000
Reactivity
Human
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1050-1150 of human DNA topoisomerase II alpha (DNA topoisomerase II alpha (TOP2A)) (NP_001058.2).
Western Blot - Anti-Topoisomerase II alpha Antibody (A91536)
Western blot analysis of extracts of various cell lines, using Anti-Topoisomerase II alpha Antibody (A91536) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.
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