Anti-Topoisomerase I Antibody [ARC0708] (A307759)

Name: Anti-Topoisomerase I Antibody [ARC0708]
Brand: Antibodies.com
SKU: A307759
Price: 485.0 USD
Availability: InStock

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$485

Rabbit monoclonal [ARC0708] antibody to Topoisomerase I for WB, IHC, ICC/IF and IP.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 5-8 business days.

Specifications

Name

Anti-Topoisomerase I Antibody [ARC0708]

Description

Rabbit monoclonal [ARC0708] antibody to Topoisomerase I.

Applications

WB, IHC, ICC/IF, IP

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200, IP: 1:500-1:1,000

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human DNA topoisomerase I (TOP1) (P11387).

Sequence

MSGDHLHNDSQIEADFRLNDSHKHKDKHKDREHRHKEHKKEKDREKSKHSNSEHKDSEKKHKEKEKTKHKDGSSEKHKDKHKDRDKEKRKEEKVRASGDA

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC0708

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

100 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

DNA topoisomerase 1, DNA topoisomerase I

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (7)

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Western Blot - Anti-Topoisomerase I Antibody [ARC0708] (A307759)

Western blot analysis of extracts of various cell lines, using Anti-Topoisomerase I Antibody [ARC0708] (A307759) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Western Blot - Anti-Topoisomerase I Antibody [ARC0708] (A307759)

Western blot analysis of extracts of Mouse thymus, using Anti-Topoisomerase I Antibody [ARC0708] (A307759) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 5s.

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Immunohistochemistry - Anti-Topoisomerase I Antibody [ARC0708] (A307759)

Immunohistochemistry analysis of paraffin-embedded human thyroid cancer using Anti-Topoisomerase I Antibody [ARC0708] (A307759) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Immunofluorescence - Anti-Topoisomerase I Antibody [ARC0708] (A307759)

Immunofluorescence analysis of C6 cells using Anti-Topoisomerase I Antibody [ARC0708] (A307759) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).