Anti-Topoisomerase I Antibody (A88019)

Name: Anti-Topoisomerase I Antibody
Brand: Antibodies.com
SKU: A88019
Price: 345.0 USD
Availability: InStock

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$345

Rabbit polyclonal antibody to Topoisomerase I for WB, IHC and IP.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 5-8 business days.

Specifications

Name

Anti-Topoisomerase I Antibody

Description

Rabbit polyclonal antibody to Topoisomerase I.

Applications

WB, IHC, IP

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, IP: 1:20-1:50

Reactivity

Human, Mouse

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human DNA topoisomerase I (DNA topoisomerase I (TOP1)) (NP_003277.1).

Sequence

MSGDHLHNDSQIEADFRLNDSHKHKDKHKDREHRHKEHKKEKDREKSKHSNSEHKDSEKKHKEKEKTKHKDGSSEKHKDKHKDRDKEKRKEEKVRASGDA

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

120 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

DNA topoisomerase 1, DNA topoisomerase I

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (3)

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Western Blot - Anti-Topoisomerase I Antibody (A88019)

Western blot analysis of extracts of various cell lines, using Anti-Topoisomerase I Antibody (A88019) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

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Immunohistochemistry - Anti-Topoisomerase I Antibody (A88019)

Immunohistochemistry analysis of paraffin-embedded mouse liver using Anti-Topoisomerase I Antibody (A88019) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Western Blot - Anti-Topoisomerase I Antibody (A88019)

Immunoprecipitation analysis of 200µg extracts of Jurkat cells using 3µg of Anti-Topoisomerase I Antibody (A88019). This Western blot was performed on the immunoprecipitate using Anti-Topoisomerase I Antibody (A88019) at a dilution of 1:1000.

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