Anti-TOP2A Antibody (A329922)

Name: Anti-TOP2A Antibody
Brand: Antibodies.com
SKU: A329922
Price: 355.0 USD
Availability: InStock

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$355

Rabbit polyclonal antibody to TOP2A for WB, IHC-P and ELISA.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-TOP2A Antibody

Description

Rabbit polyclonal antibody to TOP2A.

Applications

WB, IHC-P, ELISA

Positive Control

HeLa.

Dilutions

WB: 1:500-1:1,000, IHC-P: 1:50-1:200, ELISA: 1 µg/ml (starting concentration)

Reactivity

Human

Immunogen

A synthetic peptide corresponding to amino acids 1431-1531 of human TOP2A.

Sequence

GAKKRAAPKGTKRDPALNSGVSQKPDPAKTKNRRKRKPSTSDDSDSNFEKIVSKAVTSKKSKGESDDFHMDFDSAVAPRAKSVRAKKPIKYLEESDEDDLF

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

190 kDa

Predicted MW

174 kDa, 177 kDa, 178 kDa, 182 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% ProClin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

DNA topoisomerase 2-alpha, DNA topoisomerase II, alpha isozyme, TOP2

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (3)

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Western Blot - Anti-TOP2A Antibody (A329922)

TOP2A expression in HeLa cell lysates analyzed by western blot. Primary antibody incubation was performed for 1 hour on 25ug protein per lane with Anti-TOP2A Antibody (A329922) at a dilution of 1:400 and detected with chemiluminescence.

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Immunohistochemistry - Anti-TOP2A Antibody (A329922)

TOP2A expression in human colon carcinoma tissue analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved with 10 mM Tris-EDTA buffer, pH 9.0, under high pressure. Staining was performed with Anti-TOP2A Antibody (A329922) at a dilution of 1:100.

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Immunohistochemistry - Anti-TOP2A Antibody (A329922)

TOP2A expression in human cervix cancer tissue analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved with 10 mM Tris-EDTA buffer, pH 9.0, under high pressure. Staining was performed with Anti-TOP2A Antibody (A329922) at a dilution of 1:100.

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