Anti-TNF Receptor I Antibody (A13494)

Name: Anti-TNF Receptor I Antibody
Brand: Antibodies.com
SKU: A13494
Price: 345.0 USD
Availability: InStock

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$345

Rabbit polyclonal antibody to TNF Receptor I for WB, IHC and ICC/IF.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 5-8 business days.

Specifications

Name

Anti-TNF Receptor I Antibody

Description

Rabbit polyclonal antibody to TNF Receptor I.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:100-1:500, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 345-455 of human TNFR1/TNFRSF1A (NP_001056.1).

Sequence

AHKPQSLDTDDPATLYAVVENVPPLRWKEFVRRLGLSDHEIDRLELQNGRCLREAQYSMLATWRRRTPRREATLELLGRVLRDMDLLGCLEDIEEALCGPAALPPAPSLLR

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

55 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

CD120a, p55, p60, TNF-R1, TNF-RI, TNFAR, TNFR-I, TNFRSF1A, Tumor necrosis factor receptor 1, Tumor necrosis factor receptor superfamily member 1A, Tumor necrosis factor receptor type I

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (3)

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Western Blot - Anti-TNF Receptor I Antibody (A13494)

Western blot analysis of extracts of various cell lines, using Anti-TNF Receptor I Antibody (A13494) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

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Immunohistochemistry - Anti-TNF Receptor I Antibody (A13494)

Immunohistochemistry analysis of paraffin-embedded rat spleen using Anti-TNF Receptor I Antibody (A13494) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Immunofluorescence - Anti-TNF Receptor I Antibody (A13494)

Immunofluorescence analysis of BALB-3T3 cells using Anti-TNF Receptor I Antibody (A13494) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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