Anti-TNF alpha Antibody - BSA and Azide free (A283066)

Anti-TNF alpha Antibody - BSA and Azide free

Goat polyclonal antibody to TNF alpha.

This antibody recognises human Tumour Necrosis Factor alpha (TNF–alpha), a 17.4kDa multi-functional pro-inflammatory cytokine primarily secreted by macrophages, but also produced by T and B lymphocytes, activated monocytes and fibroblasts.TNF-alpha exists in both a transmembrane and mature soluble form and has many biological properties, including activation of transcription factor NF-kappaB, the induction of apoptosis in various tumour cell lines and the stimulation of cell differentiation and proliferation under certain conditions. TNF-alpha also plays a role in inflammation, septic shock, autoimmune disease and rheumatoid arthritis.

WB, IHC-P, ELISA, Functional Assays

ELISA: 0.25 - 1 µg/ml, Functional Assays: 0.012 - 0.014 µg/ml, IHC-P: 2.5 - 5.0 µg/ml, WB: 0.1 - 0.2 µg/ml

Human

Recombinant human TNF alpha.

Goat

Polyclonal

IgG

Lyophilized

Reconstitute with 0.1 ml of distilled water. For long term storage the addition of 0.09% sodium azide is recommended. For functional studies do not add sodium azide.

Supplied in Phosphate Buffered Saline.

Lyophilized: Store at -20°C. Reconstituted: Store undiluted at -20°C, avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.

Goat anti Human TNF alpha antibody recognizes human Tumour Necrosis Factor alpha (TNF–alpha), a 17.4kDa multi-functional pro-inflammatory cytokine primarily secreted by macrophages, but also produced by T and B lymphocytes, activated monocytes and fibroblasts.TNF-alpha exists in both a transmembrane and mature soluble form and has many biological properties, including activation of transcription factor NF-kappaB, the induction of apoptosis in various tumour cell lines and the stimulation of cell differentiation and proliferation under certain conditions. TNF-alpha also plays a role in inflammation, septic shock, autoimmune disease and rheumatoid arthritis.

Cachectin, TNF, TNF-a, TNF-alpha, TNFA, TNFSF2, Tumor necrosis factor, Tumor necrosis factor ligand superfamily member 2