Anti-TNF alpha Antibody - BSA and Azide free (A282981)

Anti-TNF alpha Antibody - BSA and Azide free

Rabbit polyclonal antibody to TNF alpha.

This antibody recognises rat tumor necrosis factor alpha (TNFa), also known as Cachectin or Tumor necrosis factor ligand superfamily member 2. Rat TNFa is a 235 amino acid ~17 kDa single pass type II O-linked transmembrane glycoprotein, it also exists as a secreted cytokine through cleavage between residues 79 and 80. TNFa is produced by many cell types including macrophages, monocytes, neutrophils and a population of activated NK cells.

WB, ELISA, IHC-P, Functional Assays

ELISA: 0.5 - 2.0 µg/ml, Functional Assays: 0.07 - 0.1 µg/ml, WB: 0.1 - 0.2 µg/ml

Rat

Recombinant rat TNF alpha.

Rabbit

Polyclonal

IgG

1 mg/ml after reconstitution.

Reconstitute with 0.1 ml of distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Gently mix after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended. For functional studies do not add sodium azide

Supplied in Phosphate Buffered Saline.

Lyophilized: Store at -20°C. Reconstituted: Store undiluted at -20°C, avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.

Rabbit anti Rat TNF alpha antibody recognizes rat tumor necrosis factor alpha (TNFa), also known as Cachectin or Tumor necrosis factor ligand superfamily member 2. Rat TNFa is a 235 amino acid ~17 kDa single pass type II O-linked transmembrane glycoprotein, it also exists as a secreted cytokine through cleavage between residues 79 and 80. TNFa is produced by many cell types including macrophages, monocytes, neutrophils and a population of activated NK cells.

Cachectin, TNF, TNF-a, TNF-alpha, TNFA, TNFSF2, Tumor necrosis factor, Tumor necrosis factor ligand superfamily member 2