General Notes
Mouse anti Human TIMP-1 antibody, clone M03, recognizes Tissue Inhibitor of Metalloproteinases 1 (TIMP-1) also known as metalloproteinase inhibitor 1, EPA and fibroblast collagenase inhibitor. TIMP-1 is a member of the TIMP family of proteins that assist in regulating the activity of matrix metalloproteinases (MMPs) and it is mainly expressed by macrophages and fibroblasts. TIMP proteins are specific endogenous inhibitors that bind to the active site of MMPs in a stoichiometric 1:1 molar ratio, thereby blocking access to ECM substrates (Kapoor et al. 2016, Robert et al. 2016). The TIMP proteins including TIMP-1 have poor specificity for any particular MMP and each can inhibit multiple MMPs with differing efficacies (Cui et al. 2017). The ratio of MMP and TIMP often determine the extent of ECM protein degradation and tissue remodeling. An imbalance of this ratio such as with decreased expression of TIMP can result in various pathological conditions, most notably, heart failure, osteoarthritis and cancer. Low levels of TIMP have also been associated with slow-to-heal wounds and venous wounds (Cui et al. 2017).The purified Mouse anti Human TIMP-1 antibody, clone M03 (MCA62678GA) can be used as a capture antibody in a sandwich ELISA with the biotinylated Mouse anti Human TIMP-1 antibody, clone M05 (MCA6279B) as the detection antibody.
