Recombinant rabbit monoclonal [DM178] antibody to TIGIT.
Applications
ELISA, Flow Cytometry
Dilutions
ELISA: 1:5,000-10,000, Flow Cytometry: 1:100
Reactivity
Human
Host
Rabbit
Clonality
Monoclonal
Clone ID
DM178
Isotype
IgG
Conjugate
Unconjugated
Purification
Affinity chromatography.
Concentration
Reconstitution dependent.
Product Form
Lyophilized
Reconstitution
Reconstitute with distilled sterile water.
Formulation
Lyophilized from sterile Phosphate Buffered Saline, pH 7.4. Normally 5%–8% Trehalose is added as a protectant before lyophilization.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
General Notes
Prior to reconstitution, centrifuge the vial at 5,000g for 3-5 minutes at room temperature. Reconstitute with appropriate volume of distilled sterile water to bring product to 1mg/ml concentration. After addition of distilled sterile water, mix by gentle tapping. Note: It is not recommended to vortex or vigorously pipette the sample.
Synonyms
T-cell immunoreceptor with Ig and ITIM domains, V-set and immunoglobulin domain-containing protein 9, V-set and transmembrane domain-containing protein 3, VSIG9, VSTM3
Flow cytometry analysis with Anti-TIGIT Antibody [DM178] - Azide free (A318531) on Expi293 cells transfected with human TIGIT (blue histogram) or Expi293 transfected with irrelevant protein (red histogram).
Anti-TIGIT Antibody [DM178] - BSA and Azide free (A318531)
Competition assay demonstrating Anti-TIGIT Antibody [DM178] - Azide free (A318531) blockade of CD155 binding to Jurkat cell line transfected with human TIGIT, and competitive activity is better than Etigilimab biosimilar.
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