Anti-Thymidine Phosphorylase Antibody [P-GF.44C] (A248382)

Anti-Thymidine Phosphorylase Antibody [P-GF.44C]

Mouse monoclonal [P-GF.44C] antibody to Thymidine Phosphorylase.

This antibody recognizes a protein (amino acid 482) of 55kDa (in vivo 110kDa homodimer), identified as platelet-derived endothelial growth factor (PD-ECGF), same as thymidine phosphorylase (TP) or gliostatin. In the presence of inorganic orthophosphate, it catalyzes the reversible phospholytic cleavage of thymidine and deoxyuridine to their corresponding bases and 2-deoxyribose-1-phosphate. It is both chemotactic and mitogenic for endothelial cells and a non-heparin binding angiogenic factor present in platelets. Its enzymatic activity is crucial for angiogenic activity (metabolite is angiogenic). Higher levels of serum TP/PD-ECGF are observed in cancer patients. It is also involved in transformation of fluoropyrimidines, cytotoxic agents used in the treatment of a variety of malignancies, into active cytotoxic metabolites (e.g. 5-deoxy-5-fluorouridine to 5-FU). High intra-cellular levels of TP/PD-ECGF are associated with increased chemosensitivity to such antimetabolites.

WB, IP, IHC-P

WB: 1-2 µg/ml, IP: 0.5-1 µg / 500 µg protein lysate, IHC-P: 1-2 µg/ml

Human, Mouse, Rat

Recombinant full-length human Thymidine Phosphorylase protein.

Mouse

Monoclonal

P-GF.44C

IgG1

Protein A/G chromatography.

200 µg/ml

55 kDa

Liquid

Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide.

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

This monoclonal antibody is also available in a different formulation without BSA and Sodium Azide - Anti-Thymidine Phosphorylase Antibody [P-GF.44C] - BSA and Azide free (A251564).

ECGF1, Gliostatin, PD-ECGF, Platelet-derived endothelial cell growth factor, TdRPase, TP, TYMP