This antibody recognises rat TGN38, a 357 amino acid single pass trans membrane glycoprotein found primarily in the trans-golgi network, and acts as an excellent marker for this cellular organelle. TGN38 is likely to have a role in intracellular transport and plays a role in morphological maintenance. It is the homologue of human TGN46 and macaque TGN47.
Applications
WB, IHC-Fr, IF
Dilutions
IHC-Fr: 0.1 µg/ml - 1 µg/ml, WB: 0.1 µg/ml - 1 µg/ml, Fixation with methanol or methanol/acetone recommended.
Reactivity
Rat, Mouse
Immunogen
Recombinant fusion protein corresponding to extracellular domain of TGN38.
Host
Sheep
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Concentration
100 µg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 0.5% BSA, 25% Glycerol, and <0.1% Sodium Azide.
Storage
Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.
General Notes
Sheep anti Rat TGN38 antibody recognizes rat TGN38, a 357 amino acid single pass trans membrane glycoprotein found primarily in the trans-golgi network, and acts as an excellent marker for this cellular organelle (Humphrey et al. 1993).TGN38 is likely to have a role in intracellular transport (McNamara et al. 2004) and plays a role in morphological maintenance (Girotti and Banting 1996). It is the homologue of human TGN46 and macaque TGN47 (Ponnambalam et al. 1996).