This antibody recognises rat TGN38, a 357 amino acid single pass trans membrane glycoprotein found primarily in the trans-golgi network, and acts as an excellent marker for this cellular organelle. TGN38 is likely to have a role in intracellular transport and plays a role in morphological maintenance. It is the homologue of human TGN46 and macaque TGN47.
Applications
WB, IHC-Fr, IF, Electron Microscopy
Dilutions
IHC-Fr: 1:100 - 1:200, WB: 1:1,000, Fixation with methanol or methanol/acetone recommended.
Reactivity
Rat, Mouse
Immunogen
Recombinant fusion protein corresponding to extracellular domain of TGN38.
Host
Sheep
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Concentration
Lot Specific
Purity
Diluted Serum.
Product Form
Liquid
Formulation
Supplied as diluted serum in Phosphate Buffered Saline with 25% Glycerol, 1% BSA, and <0.1% Sodium Azide
Storage
Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.
General Notes
Sheep anti Rat TGN38 antibody recognizes rat TGN38, a 357 amino acid single pass trans membrane glycoprotein found primarily in the trans-golgi network, and acts as an excellent marker for this cellular organelle (Humphrey et al. 1993).TGN38 is likely to have a role in intracellular transport (McNamara et al. 2004) and plays a role in morphological maintenance (Girotti and Banting 1996). It is the homologue of human TGN46 and macaque TGN47 (Ponnambalam et al. 1996).