This antibody recognizes a monomorphic determinant of TCR alpha/beta, the dominant subtype of T cell receptor expressed in human peripheral blood.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: The reagent is designed for analysis of human blood cells using 4 µl reagent / 100 µl of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Reactivity
Human
Immunogen
Unfortunately, this information is considered to be commercially sensitive.
Host
Mouse
Clonality
Monoclonal
Clone ID
IP26
Isotype
IgG1
Conjugate
PE-Cyanine 5
Excitation: 496nm, Emission: 667nm
Purification
This antibody is conjugated with tandem dye PE-Cyanine 5 under optimum conditions. The conjugate is purified by size-exclusion chromatography; removing unconjugated antibody and free fluorochrome.
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Store in the dark at 2-8°C. Do not freeze!
Separation of human TCR alpha/beta positive lymphocytes (red-filled) from TCR alpha/beta negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained with Anti-TCR alpha + TCR beta Antibody [IP26] (PE-Cyanine 5) (4 µl reagent / 100 µl of peripheral whole blood).
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