Hamster monoclonal [H57-597] antibody to TCR alpha/beta.
Specificity
This antibody recognises the beta-chain of the murine alpha/beta T cell receptor and does not show any reactivity to TCR gamma/delta (Gascoigne 1990).Clone H57-597 has been reported to activate T-cells in immobilized form and has also been used for in vivo cell depletion (van der Heyde et al., 1995 and Skeen & Ziegler 1993). Removal of sodium azide is recommended prior to using this clone in functional assays.
Applications
Flow Cytometry, IP
Dilutions
Flow Cytometry: 0.2-1ug/106 cells
Reactivity
Mouse
Immunogen
Affinity purified murine T cell receptor.
Host
Hamster
Clonality
Monoclonal
Clone ID
H57-597
Isotype
IgG
Conjugate
Unconjugated
Purification
Protein A affinity chromatography of tissue culture supernatant.
Concentration
1 mg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.
Storage
Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.
General Notes
Hamster anti mouse TCR a/ß, clone H57-597, recognizes the ß-chain of the murine a/ß T cell receptor and does not show any reactivity to TCR?/d (Gascoigne 1990).Clone H57-597 has been reported to activate T-cells in immobilized form (Kubo et al. 1989) and has also been used for in vivo cell depletion (van der Heyde et al., 1995 and Skeen & Ziegler 1993). Removal of sodium azide is recommended prior to using this clone in functional assays.
