Western Blot - Anti-Tau (phospho Thr231) Antibody [ARC0021] (A309486)
Western blot analysis of extracts of various cell lines, using Anti-Tau (phospho Thr231) Antibody [ARC0021] (A309486) at 1:1,000 dilution. HeLa and NIH/3T3 cells were treated by CIP(20uL/400ul) at 37°C for 1 hour. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3s.
Immunofluorescence analysis of mouse brain using Anti-Tau (phospho Thr231) Antibody [ARC0021] (A309486). DAPI was used to stain the cell nuclei (blue).
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