Western Blot - Anti-Tau (phospho Thr181) Antibody (A90177)
Western blot analysis of extracts of HeLa cells, using Anti-Tau (phospho Thr181) Antibody (A90177) at 1:1,000 dilution. HeLa cells were treated by Calyculin A (100 nM) at 37°C for 30 minutes after serum-starvation overnight. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 90s.
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