Anti-Tau (phospho Ser416) Antibody (A308895)

Name: Anti-Tau (phospho Ser416) Antibody
Brand: Antibodies.com
SKU: A308895
Price: 485.0 USD
Availability: InStock

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$485

Rabbit polyclonal antibody to Tau (phospho Ser416) for WB, IHC and ICC/IF.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Tags:

Cytoplasm Marker Cytoskeleton Marker Mitochondrial Marker Plasma Membrane Marker

Specifications

Name

Anti-Tau (phospho Ser416) Antibody

Description

Rabbit polyclonal antibody to Tau (phospho Ser416).

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic phosphorylated peptide around S416 of human Tau (NP_005901.2).

Sequence

TGSID

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

95 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

MAPT, MAPTL, Microtubule-associated protein tau, MTBT1, Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (4)

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Western Blot - Anti-Tau (phospho Ser416) Antibody (A308895)

Western blot analysis of extracts of NIH/3T3 cells, using Anti-Tau (phospho Ser416) Antibody (A308895) at 1:1,000 dilution or Tau antibody (A0002). NIH/3T3 cells were treated by CIP(20uL/400ul) at 37°C for 1 hour. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA. Detection was with a ECL Basic Kit. Exposure time: 60s.

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Immunohistochemistry - Anti-Tau (phospho Ser416) Antibody (A308895)

Immunohistochemistry analysis of paraffin-embedded human brain tissue using Anti-Tau (phospho Ser416) Antibody (A308895) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Immunofluorescence - Anti-Tau (phospho Ser416) Antibody (A308895)

Immunofluorescence analysis of rat brain using Anti-Tau (phospho Ser416) Antibody (A308895) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue).

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Immunofluorescence - Anti-Tau (phospho Ser416) Antibody (A308895)

Immunofluorescence analysis of mouse brain using Anti-Tau (phospho Ser416) Antibody (A308895) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue).

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