Western Blot - Anti-Tau (phospho Ser404) Antibody (A16489)
Western blot analysis of extracts of SH-SY5Y and C6 cells, using Anti-Tau (phospho Ser404) Antibody (A16489) at 1:2,000 dilution. SH-SY5Y cells were treated by Okadaic Acid (100nM) for 1 hour and Calyculin A (100nM) for 30 minutes after serum-starvation overnight. C6 cell lysates were treated by CIP at 37°C for 1 hour. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA. Detection was with a ECL Basic Kit. Exposure time: 3s.
Immunohistochemistry analysis of paraffin-embedded rat hippocampal region tissue from a model with Alzheimer, using Anti-Tau (phospho Ser404) Antibody (A16489). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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