Anti-Tau (phospho Ser396) Antibody [ARC1573] (A309489)

Western blot analysis of extracts of Mouse brain, using Anti-Tau (phospho Ser396) Antibody [ARC1573] (A309489) at 1:1,000 dilution. Mouse brain were treated by CIP(20uL/400ul) at 37°C for 1 hour. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA. Detection was with a ECL Basic Kit. Exposure time: 90s.

Immunohistochemistry analysis of paraffin-embedded rat brain using Anti-Tau (phospho Ser396) Antibody [ARC1573] (A309489) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunohistochemistry analysis of paraffin-embedded human brain tissue using Anti-Tau (phospho Ser396) Antibody [ARC1573] (A309489) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunohistochemistry analysis of paraffin-embedded mouse brain using Anti-Tau (phospho Ser396) Antibody [ARC1573] (A309489) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.