Western Blot - Anti-Tau (phospho Ser262) Antibody (A16624)
Western blot analysis of extracts of Mouse brain, using Anti-Tau (phospho Ser262) Antibody (A16624) at 1:500 dilution. Mouse brain were treated by CIP(20uL/400ul) at 37°C for 1 hour. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 120s.
Immunohistochemistry analysis of paraffin-embedded rat hippocampal region tissue from a model with Alzheimer using Anti-Tau (phospho Ser262) Antibody (A16624). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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