Western Blot - Anti-Tau (phospho Ser246 + Ser259 + Ser220) Antibody (A308549)
Western blot analysis of extracts of NIH/3T3 cells, using Anti-Tau (phospho Ser246 + Ser259 + Ser220) Antibody (A308549) at 1:1,000 dilution or Tau antibody (A0002). NIH/3T3 cells were treated by CIP(20uL/400ul) at 37°C for 1 hour. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA. Detection was with a ECL Basic Kit. Exposure time: 120s.
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