Anti-Tau Antibody (A85414)

Immunofluorescent analysis of cortical neuron-glial culture from E20 rat stained with Anti-Tau Antibody, at a dilution of 1:2,000, in green, and Anti-MAP2 Antibody (A85297 | 1:1,000, in red. The blue is DAPI staining of nuclear DNA. The Anti-Tau Antibody stains perikarya, dendrites and axons of neurons, while the Anti-MAP2 Antibody labels only dendrites and perikarya. As a result, perikarya and dendrites appear orange-yellow, since they contain both MAP2 and tau proteins.

We obtained E18 hippocampal neurons and grew them for seven days. We fixed and immunostained with Anti-Tau Antibody (red channel) and Anti-UCHL1 Antibody (A85351 | green). Ubquitin C-terminal Hydrolase 1 (UCHL1) is an abundant cytoplasmic protein of neurons which is concentrated in the perikarya. Since the perikarya contain both UCHL1 and Tau, the red and green signals superimpose, giving a yellow color, while the processes, which contain relatively much more tau, appear red. Blue stain is DAPI and reveals cell nuclei of some non neuronal cells in these cultures.

Western blot analysis of tissue lysates using Anti-Tau Antibody, at a dilution of 1:10,000, in green,: [Lane 1] protein standard (red), [Lane 2] rat brain, [Lane 3] rat spinal cord, [Lane 4] mouse brain, [Lane 5] mouse spinal cord. Tau protein is expressed as multiple isoforms of different molecular weight, and so appears as multiple closely spaced bands in the region of the blot in the range from 48 kDa to 67 kDa.

Immunohistochemistry analysis of a formalin fixed paraffin embedded human cortical section. Tissue from an Alzheimer’s disease patient was stained with Anti-Tau Antibody (A85414) at a dilution of 1:10,000, and detected using DAB (brown) using the ABC method. Counterstained with Hematoxylin (blue). The Anti-Tau Antibody (A85414) labels neurofibrillary tangles in the cytoplasm of diseased neurons producing a characteristic flame shaped morphology and also highlights dystrophic neurites associated with senile plaques.