Western blot analysis of SH-SY5Y showing detection of ~45.8 kDa Tau protein using Anti-Tau Antibody (A304914) at 1:1,000 for 2 hours at room temperature with shaking. Lane 1: Molecular Weight Ladder (MW). Lane 2: SH-SY5Y. Load: 10 µg. Block: 5% Skim Milk powder in TBST. The secondary antibody used was Goat anti-rabbit IgG:HRP at 1:4,000 for 1 hour at room temperature with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 minutes in room temperature. Predicted/Observed Size: ~45.8 kDa. Other Band(s): 50 kDa.
Immunohistochemistry analysis of human brain cortex, fixed in formalin and paraffin-embedded. The Primary Antibody used was Anti-Tau Antibody (A304914).
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