Anti-Tau Antibody [5B10] (A85415)

Name: Anti-Tau Antibody [5B10]
Brand: Antibodies.com
SKU: A85415
Price: 190.0 USD
Availability: InStock

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$190

Mouse monoclonal [5B10] antibody to Tau for WB, ICC/IF and IHC.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 5-8 business days.

Tags:

Cytoplasm Marker Cytoskeleton Marker Mitochondrial Marker Plasma Membrane Marker

Specifications

Name

Anti-Tau Antibody [5B10]

Description

Mouse monoclonal [5B10] antibody to Tau.

Specificity

The epitope for this antibody is located in the peptide HVPGGGNKKIETHKLTFREN, amino acids 362-381 of human Tau (NP_005901.2). This sequence is expressed in all known human Tau isotypes and is totally conserved in all mammals and other higher vertebrates.

Applications

WB, ICC/IF, IHC

Dilutions

WB: 1:10,000, ICC/IF: 1:1,000, IHC: 1:10,000

Reactivity

Human, Rat, Mouse

Immunogen

Recombinant full-length version of one of the lower molecular weight human Tau isoforms, specifically the human 441 amino acid htau40 form described by Goedert et al., expressed in and purified from E. coli.

Host

Mouse

Clonality

Monoclonal

Clone ID

5B10

Isotype

IgG1

Conjugate

Unconjugated

Purification

Immunogen affinity purification.

Concentration

1 mg/ml

Molecular Weight

48-67 kDa, with higher molecular weight "big tau" isoforms mostly in peripheral tissues.

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline with 50% Glycerol and 5mM Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

MAPT, MAPTL, Microtubule-associated protein tau, MTBT1, Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau

Isotype Controls

Suitable Secondaries

See all Anti-Mouse IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (6)

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Immunofluorescence - Anti-Tau Antibody [5B10] (A85415)

Immunofluorescent analysis of cortical neuron-glial culture from E20 rat stained with Anti-Tau Antibody, at a dilution of 1:2,000, in green, and Anti-GFAP Antibody (A85307 | 1:5,000, in red. The blue is DAPI staining of nuclear DNA. Anti-Tau Antibody stains perikarya, dendrites and axons of neurons, while the Anti-GFAP Antibody labels astrocytes.

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Immunofluorescence - Anti-Tau Antibody [5B10] (A85415)

E18 rat brain cultures were grown for 10 days, fixed and immunostained with Anti-Tau Antibody (red), Anti-MAP2 Antibody (A85363 | green) and for DNA (blue). Anti-Tau Antibody stains the neuronal perikarya, dendrites and axonal process strongly and does not stain non-neuronal cells in these cultures. The Anti-MAP2 Antibody also stains neurons only, but only the perikarya and dendrites. As a result perikarya and dendrites appears yellow, since they contain both tau and MAP2, while axons appear red as they contain only tau.

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Western Blot - Anti-Tau Antibody [5B10] (A85415)

Western blot analysis of tissue lysates using Anti-Tau Antibody, at a dilution of 1:2,000, in green,: [Lane 1] protein standard (red), [Lane 2] rat brain, [Lane 3] rat spinal cord, [Lane 4] mouse brain, [Lane 5] mouse spinal cord. Tau protein is expressed as up to 9 different isoforms of different molecular weight, and so appears as multiple closely spaced bands in the region of the blot from 48 kDa to 67 kDa with some higher molecular weight “big tau” Isoforms.

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Immunofluorescence - Anti-Tau Antibody [5B10] (A85415)

Immunofluorescent analysis of cortical neuron-glial cell culture from E20 rat costained with Anti-GFAP Antibody (A85307) at a dilution of 1:2,000 (red) and Anti-Tau Antibody [5B10] (A85415) at a dilution of 1:2,000 (green). Nuclei were stained with DAPI (blue). The GFAP labels astroglial cells, while the MAP-t antibody stains neuronal cell perikarya, dendrites and axons.