Anti-Tau Antibody [3D4] (A305069)

Immunocytochemistry/Immunofluorescence analysis of human iPSC-derived neurons, fixed in 4% PFA, using Anti-Tau Antibody [3D4] (A305069), at 1:100 for Overnight at 4°C. Counterstain: DAPI at 1:5,000 for 5 minutes at room temperature in the dark. Magnification: 40X.

Dot blot analysis of human recombinant protein using Anti-Tau Antibody [3D4] (A305069) at 1:1,000 for 2 hours at room temperature with shaking. The secondary antibody used was Goat anti-mouse IgG:HRP at 1:5,000 for 1 hour at room temperature with shaking.

Western blot analysis of mouse brain showing detection of Tau protein using Anti-Tau Antibody [3D4] (A305069) at 1:1,000 for 2 hours at room temperature with shaking. Lane 1: MW Marker. Lane 2: mouse brain (20ug). Block: 5% Skim Milk powder in TBST. The secondary antibody used was Goat anti-mouse IgG:HRP at 1:5,000 for 1 hour at room temperature with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 minutes in room temperature.