This antibody detects multiple bands. This antibody detects monomer under denaturing conditions but preferentially This antibody detects fibril under native conditions (dot blot).
Applications
WB, Dot, ICC/IF, ELISA
Dilutions
WB: 1:1,000
Reactivity
Human, Mouse, Rat
Immunogen
Human Recombinant Tau441 (2N4R), P301S mutant Protein Pre-formed Fibrils.
Host
Mouse
Clonality
Monoclonal
Clone ID
1D5
Isotype
IgG1
Conjugate
Unconjugated
Purification
Protein G purification.
Concentration
1 mg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 50% Glycerol and 0.09% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Western blot analysis of human Breast Cancer Cell line and mouse brain showing detection of Tau protein using Anti-Tau Antibody [1D5] (A305068) at 1:1,000 for 2 hours at room temperature with shaking. Lane 1: MW Marker. Lane 2: human T-47d (10ug). Lane 3: mouse brain (20ug). Block: 5% Skim Milk powder in TBST. The secondary antibody used was Goat anti-mouse IgG:HRP at 1:5,000 for 1 hour at room temperature with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 minutes in room temperature.
Immunocytochemistry/Immunofluorescence analysis of human iPSC-derived neurons, fixed in 4% PFA, using Anti-Tau Antibody [1D5] (A305068), at 1:100 for Overnight at 4°C. Counterstain: DAPI at 1:5,000 for 5 minutes at room temperature in the dark. Magnification: 40X.
Dot blot analysis of human recombinant protein using Anti-Tau Antibody [1D5] (A305068) at 1:1,000 for 2 hours at room temperature with shaking. The secondary antibody used was Goat anti-mouse IgG:HRP at 1:5,000 for 1 hour at room temperature with shaking.
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![Western Blot - Anti-Tau Antibody [1D5] (A305068) - Antibodies.com](https://cdn.antibodies.com/image/catalog/305/A305068_1.png?profile=product_top)
![Immunocytochemistry/Immunofluorescence - Anti-Tau Antibody [1D5] (A305068) - Antibodies.com](https://cdn.antibodies.com/image/catalog/305/A305068_2.png?profile=product_top)
![Dot Blot - Anti-Tau Antibody [1D5] (A305068) - Antibodies.com](https://cdn.antibodies.com/image/catalog/305/A305068_3.png?profile=product_top)
![Western Blot - Anti-Tau Antibody [1D5] (A305068) - Antibodies.com](https://cdn.antibodies.com/image/catalog/305/A305068_1.png?profile=product_top_thumb)
![Immunocytochemistry/Immunofluorescence - Anti-Tau Antibody [1D5] (A305068) - Antibodies.com](https://cdn.antibodies.com/image/catalog/305/A305068_2.png?profile=product_top_thumb)
![Dot Blot - Anti-Tau Antibody [1D5] (A305068) - Antibodies.com](https://cdn.antibodies.com/image/catalog/305/A305068_3.png?profile=product_top_thumb)
![Western Blot - Anti-Tau Antibody [1D5] (A305068) - Antibodies.com](https://cdn.antibodies.com/image/catalog/305/A305068_1.png?profile=product_image)
![Immunocytochemistry/Immunofluorescence - Anti-Tau Antibody [1D5] (A305068) - Antibodies.com](https://cdn.antibodies.com/image/catalog/305/A305068_2.png?profile=product_image)
![Dot Blot - Anti-Tau Antibody [1D5] (A305068) - Antibodies.com](https://cdn.antibodies.com/image/catalog/305/A305068_3.png?profile=product_image)
![Immunohistochemistry - Anti-Tau (phospho Ser202 + Thr205) Antibody [AH36] (A304919) - Antibodies.com](https://cdn.antibodies.com/image/catalog/304/A304919_1.png?profile=product_alternative)
![Immunocytochemistry/Immunofluorescence - Anti-Tau Antibody [3D4] (A305069) - Antibodies.com](https://cdn.antibodies.com/image/catalog/305/A305069_1.png?profile=product_alternative)
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