Anti-Syntaxin 1a Antibody [ARC2403] (A307451)

Name: Anti-Syntaxin 1a Antibody [ARC2403]
Brand: Antibodies.com
SKU: A307451
Price: 485.0 USD
Availability: InStock

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$485

Rabbit monoclonal [ARC2403] antibody to Syntaxin 1a for WB, IHC and ICC/IF.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-Syntaxin 1a Antibody [ARC2403]

Description

Rabbit monoclonal [ARC2403] antibody to Syntaxin 1a.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human STX1A (Q16623).

Sequence

MKDRTQELRTAKDSDDDDDVAVTVDRDRFMDEFFEQVEEIRGFIDKIAENVEEVKRKHSAILASPNPDEKTKEELEELMSDIKKTANKVRSKLKSIEQSI

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC2403

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

33 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

Neuron-specific antigen HPC-1, STX1, STX1A, Syntaxin-1A

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (5)

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Western Blot - Anti-Syntaxin 1a Antibody [ARC2403] (A307451)

Western blot analysis of extracts of Rat brain, using Anti-Syntaxin 1a Antibody [ARC2403] (A307451) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 5s.

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Western Blot - Anti-Syntaxin 1a Antibody [ARC2403] (A307451)

Western blot analysis of extracts of various cell lines, using Anti-Syntaxin 1a Antibody [ARC2403] (A307451) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.

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Immunohistochemistry - Anti-Syntaxin 1a Antibody [ARC2403] (A307451)

Immunohistochemistry analysis of paraffin-embedded mouse brain using Anti-Syntaxin 1a Antibody [ARC2403] (A307451) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Immunofluorescence - Anti-Syntaxin 1a Antibody [ARC2403] (A307451)

Immunofluorescence analysis of rat brain using Anti-Syntaxin 1a Antibody [ARC2403] (A307451) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).