Rabbit polyclonal antibody to Synaptotagmin (Phospho-Thr202)
Specificity
The antibody detects endogenous levels of Synaptotagmin only when phosphorylated at threonine 202.
Applications
WB, IHC
Dilutions
Western blotting: 1:500~1:1000Immunohistochemistry: 1:50~1:100
Reactivity
Human, Mouse, Rat
Immunogen
Peptide sequence around phosphorylation site of threonine 202(R-K-T(p)-L-N) derived from Human Synaptotagmin.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1 mg/ml
Formulation
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage
Store at -20˚C / 1 year
Synonyms
KIAA1342, Synaptotagmin II, Synaptotagmin III, Synaptotagmin IV, Synaptotagmin V, Synaptotagmin-2, Synaptotagmin-3, Synaptotagmin-4, Synaptotagmin-5, SYT2, SYT3, SYT4, SYT5, SytII, SytIII, SytIV, SytV
Western blot analysis of extracts from colo cells (Lane 2), using Synaptotagmin (Phospho-Thr202) Antibody #11645. The lane on the left is treated with antigen-specific peptide.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Synaptotagmin (phospho-Thr202) antibody #11645 (left)or the same antibody preincubated with blocking peptide (right).
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