Rabbit polyclonal antibody to SYK (Phospho-Tyr352)
Specificity
The antibody detects endogenous levels of SYK only when phosphorylated at tyrosine 352.
Applications
WB
Dilutions
Western blotting: 1:500~1:1000
Reactivity
Human
Immunogen
Peptide sequence around phosphorylation site of tyrosine 352(S-P-Y(p)-A-D) derived from Human SYK.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1 mg/ml
Formulation
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Western blot analysis of extracts from HuvEc cells (Lane 2) and HepG2 cells (Lane 3), using SYK (Phospho-Tyr352) Antibody #11827. The lane on the left is treated with antigen-specific peptide.
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