Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
ChET 9 protein, CHET9, Chromatin precipitated E2F target 9 protein, JJAZ1, Joined to JAZF1 protein, KIAA0160, Polycomb protein SUZ12, Suppressor of zeste 12 protein homolog
Western blot analysis of extracts of various cell lines, using Anti-SUZ12 Antibody (A12169) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.
Western blot analysis of extracts from normal (control) and SUZ12 knockout (KO) HeLa cells, using Anti-SUZ12 Antibody (A12169) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.
Immunofluorescence analysis of C6 cells using Anti-SUZ12 Antibody (A12169) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
Immunoprecipitation analysis of 200µg extracts of HeLa cells using 3µg of Anti-SUZ12 Antibody (A12169). This Western blot was performed on the immunoprecipitate using Anti-SUZ12 Antibody (A12169) at a dilution of 1:500.
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