Anti-Superoxide Dismutase 1 Antibody (A84197)

Superoxide Dismutase 1 expression in Mouse Brain lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-Superoxide Dismutase 1 Antibody (A84197) at 0.02µg/ml and detected by chemiluminescence.

Superoxide Dismutase 1 expression in NIH3T3 (A), HEK293 (B), HepG2 (C), and MCF7 (D) cell lysates analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-Superoxide Dismutase 1 Antibody (A84197) at 0.01µg/ml and detected by chemiluminescence.

Superoxide Dismutase 1 expression in U2OS cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-Superoxide Dismutase 1 Antibody (A84197) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Cytoplasmic and nuclear staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.

Superoxide Dismutase 1 expression in A431 cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-Superoxide Dismutase 1 Antibody (A84197) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Cytoplasmic staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.