Western Blot - Anti-Superoxide Dismutase 1 Antibody (A305066)
Western blot analysis of Human, rat, mouse Hela cells, Brain, lung showing detection of ~18 kDa SOD1 (UBB) protein using Anti-Superoxide Dismutase 1 Antibody (A305066) at 1:1,000. Lane 1: Molecular Weight Ladder. Lane 2: human HeLa Cell Lysates. Lane 3: rat Brain. Lane 4: mouse Lung. Load: 20 µg. Predicted/Observed Size: ~18 kDa.
Immunocytochemistry/Immunofluorescence analysis of human cervical cancer cell line (HeLa), fixed in 2% formaldehyde for 20 minutes at room temperature, using Anti-Superoxide Dismutase 1 Antibody (A305066), at 1:110 for 12 hours at 4°C. The secondary antibody used was R-PE Goat Anti-Rabbit (yellow) at 1:200 for 2 hours at room temperature. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at room temperature. Localization: Cytoplasm. Nucleus. Mitochondrion. Magnification: 100x.(A) DAPI (blue) nuclear stain. (B) Anti-SOD1 (UBB) Antibody. (C) Composite.
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