Western Blot - Anti-Sumo 1 Antibody [ARC0215] (A307733)
Western blot analysis of extracts of various cell lines, using Anti-Sumo 1 Antibody [ARC0215] (A307733) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 60s.
Western Blot - Anti-Sumo 1 Antibody [ARC0215] (A307733)
Western blot analysis of extracts of various cell lines, using Anti-Sumo 1 Antibody [ARC0215] (A307733) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.
Western Blot - Anti-Sumo 1 Antibody [ARC0215] (A307733)
Western blot analysis of extracts of various cell lines, using Anti-Sumo 1 Antibody [ARC0215] (A307733) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1min.
Western Blot - Anti-Sumo 1 Antibody [ARC0215] (A307733)
Western blot analysis of extracts from wild type(WT) and SUMO1 knockout (KO) 293T cells, using Anti-Sumo 1 Antibody [ARC0215] (A307733) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.
Immunohistochemistry analysis of paraffin-embedded human breast cancer tissue using Anti-Sumo 1 Antibody [ARC0215] (A307733) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunofluorescence analysis of U-2 OS cells using Anti-Sumo 1 Antibody [ARC0215] (A307733) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
Immunofluorescence analysis of C6 cells using Anti-Sumo 1 Antibody [ARC0215] (A307733) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
Immunofluorescence analysis of NIH-3T3 cells using Anti-Sumo 1 Antibody [ARC0215] (A307733) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
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