Western Blot - Anti-Stathmin 1 (phospho Ser25) Antibody (A16523)
Western blot analysis of various lysates, using Anti-Stathmin 1 (phospho Ser25) Antibody (A16523) at 1:400 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 60s.
Western Blot - Anti-Stathmin 1 (phospho Ser25) Antibody (A16523)
Western blot analysis of various lysates, using Anti-Stathmin 1 (phospho Ser25) Antibody (A16523) at 1:400 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 60s.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using Anti-Stathmin 1 (phospho Ser25) Antibody (A16523). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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