Rabbit polyclonal antibody to STAT6 (Phospho-Thr645)
Specificity
The antibody detects endogenous level of STAT6 only when phosphorylated at threonine 645.
Applications
WB, IHC
Dilutions
Immunohistochemistry: 1:50~1:100
Reactivity
Human
Immunogen
Peptide sequence around phosphorylation site of threonine 645 (P-A-T(p)-I-K) derived from Human STAT6.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1 mg/ml
Predicted MW
110kd
Formulation
Supplied at 1.0mg / mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage
Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Synonyms
IL-4 Stat, Signal transducer and activator of transcription 6
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using STAT6 (Phospho-Thr645) Antibody #11051 (left) or the same antibody preincubated with blocking peptide (right).
Western blot analysis of extracts from Hela cells, treated with IL-4 or calf intestinal phosphatase (CIP), using STAT6 (Phospho-Thr645) Antibody #11051.
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