Anti-STAT5 (phospho Tyr694) Antibody (A91399)

Name: Anti-STAT5 (phospho Tyr694) Antibody
Brand: Antibodies.com
SKU: A91399
Price: 485.0 USD
Availability: InStock

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$485

Rabbit polyclonal antibody to STAT5 (phospho Tyr694) for WB and IHC.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-STAT5 (phospho Tyr694) Antibody

Description

Rabbit polyclonal antibody to STAT5 (phospho Tyr694).

Applications

WB, IHC

Dilutions

WB: 1:500-1:2,000, IHC: 1:100-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic phosphorylated peptide around Y694 of human STAT5 (NP_003143.2/NP_036580.2).

Sequence

DGYVK

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

90 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

Signal transducer and activator of transcription 5A

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (5)

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Western Blot - Anti-STAT5 (phospho Tyr694) Antibody (A91399)

Western blot analysis of extracts of K562 cells, using Anti-STAT5 (phospho Tyr694) Antibody (A91399) at 1:1,000 dilution. K-562 cells were treated by ATP(5 mM) at 30°C for 1 hour. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Western Blot - Anti-STAT5 (phospho Tyr694) Antibody (A91399)

Western blot analysis of extracts of A-431 cells, using Anti-STAT5 (phospho Tyr694) Antibody (A91399) at 1:1,000 dilution or STAT5B antibody (A12356). A-431 cells were treated by EGF (100 ng/ml) at 37°C for 30 minutes after serum-starvation overnight. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA. Detection was with a ECL Basic Kit. Exposure time: 60s.

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Immunohistochemistry - Anti-STAT5 (phospho Tyr694) Antibody (A91399)

Immunohistochemistry analysis of paraffin-embedded rat heart using Anti-STAT5 (phospho Tyr694) Antibody (A91399) at a dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Immunohistochemistry - Anti-STAT5 (phospho Tyr694) Antibody (A91399)

Immunohistochemistry analysis of paraffin-embedded human placenta using Anti-STAT5 (phospho Tyr694) Antibody (A91399) at a dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.