Recombinant rabbit monoclonal [RM261] antibody to STAT3 (phospho Tyr705).
Specificity
This antibody recognizes human STAT3 only when phosphorylated at Tyr705.
Applications
WB, IHC
Dilutions
IHC: 1:1,000-1:10,000, WB: 1:1,000-1:2,000
Reactivity
Human
Cross Reactivity
This antibody does not cross-react with STAT3 without phosphorylation at Tyr705. This antibody may react with samples from mouse and rat, as predicted by immunogen homology.
Immunogen
Synthetic peptide corresponding to human STAT3 (phospho Tyr705).
Host
Rabbit
Clonality
Monoclonal
Clone ID
RM261
Isotype
IgG
Conjugate
Unconjugated
Purification
Protein A chromatography.
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 50% Glycerol, 1% BSA, and 0.09% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
Acute-phase response factor, APRF, Signal transducer and activator of transcription 3
Western Blot - Anti-STAT3 (phospho Tyr705) Antibody [RM261] (A121192)
Western blot of A431 cell lysates, non-treated (-) or treated (+) with EGF, using Anti-STAT3 (phospho Tyr705) Antibody [RM261] at a 1:1,000 dilution, showed STAT3 (phospho Tyr705) in EGF treated A431 cells.
Immunohistochemical staining of formalin fixed and paraffin embedded human breast cancer tissue sections using Anti-STAT3 (phospho Tyr705) Antibody [RM261] at a 1:10,000 dilution.
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