Western Blot - Anti-STAT3 (phospho Tyr705) Antibody (A16431)
Western blot analysis of extracts of HeLa cells, using Anti-STAT3 (phospho Tyr705) Antibody (A16431) at 1:500 dilution. HeLa cells were treated by IFN-alpha (100 ng/ml) at 37°C for 30 minutes after serum-starvation overnight. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.
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