Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Synonyms
BHLHD2, Class D basic helix-loop-helix protein 2, SREBF2, SREBP-2, Sterol regulatory element-binding protein 2, Sterol regulatory element-binding transcription factor 2
Western blot analysis of various lysates, using Anti-SREBP2 Antibody (A90894) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 20s.
Immunohistochemistry analysis of paraffin-embedded human liver cancer using Anti-SREBP2 Antibody (A90894) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded mouse spinal cord using Anti-SREBP2 Antibody (A90894) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
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