Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
BHLHD1, Class D basic helix-loop-helix protein 1, SREBF1, SREBP-1, Sterol regulatory element-binding protein 1, Sterol regulatory element-binding transcription factor 1
Western blot analysis of various lysates, using Anti-SREBP1 Antibody (A88065) at 1:2,500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 60s.
Immunofluorescence analysis of HepG2 cells using Anti-SREBP1 Antibody (A88065) at a dilution of 1:20 (40x lens). DAPI was used to stain the cell nuclei (blue).
Immunofluorescence analysis of NIH/3T3 cells using Anti-SREBP1 Antibody (A88065) at a dilution of 1:20 (40x lens). DAPI was used to stain the cell nuclei (blue).
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