Rabbit polyclonal antibody to SREBP-1 (phospho Ser439).
Specificity
This antibody detects endogenous levels of SREBP-1 only when phosphorylated at Ser439.
Applications
WB, IHC, ELISA
Dilutions
WB: 1:500-1:1000, ELISA: 1:10000
Reactivity
Human, Rat
Immunogen
Synthetic peptide derived from human SREBP-1 around the phosphorylation site of Ser439 (amino acids 405-454).
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Purification
Purified from rabbit serum by antigen affinity chromatography using the immunizing phospho peptide.
Molecular Weight
121kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline (without Mg2+ and Ca2+), pH 7.4, with 150mM NaCl, 0.02% Sodium Azide, and 50% Glycerol.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
BHLHD1, Class D basic helix-loop-helix protein 1, SREBF1, SREBP-1, Sterol regulatory element-binding protein 1, Sterol regulatory element-binding transcription factor 1
Immunohistochemical analysis of paraffin-embedded human brain using Anti-SREBP-1 (phospho Ser439) Antibody. The right hand panel represents a negative control, where the antibody was pre-incubated with the immunising peptide.
Western Blot - Anti-SREBP-1 (phospho Ser439) Antibody (A93802)
Western blot analysis of lysates from Jurkat cells treated with TNF 20ng/ml 30' using Anti-SREBP-1 (phospho Ser439) Antibody. The right hand lane represents a negative control, where the antibody is blocked by the immunising peptide.
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