Anti-Src (phospho Tyr529) Antibody (A16498)

Name: Anti-Src (phospho Tyr529) Antibody
Brand: Antibodies.com
SKU: A16498
Price: 485.0 USD
Availability: InStock

Enlarge Image

$485

Rabbit polyclonal antibody to Src (phospho Tyr529) for WB, IHC and ICC/IF.

100% Guarantee

Price Match Guarantee

Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-Src (phospho Tyr529) Antibody

Description

Rabbit polyclonal antibody to Src (phospho Tyr529).

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:100, ICC/IF: 1:100-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic phosphorylated peptide around Y529 of human Src (NP_005408.1).

Sequence

EPQYQ

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

60 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

p60-Src, pp60c-src, Proto-oncogene c-Src, Proto-oncogene tyrosine-protein kinase Src, SRC1

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (1)

Enlarge Image

Western Blot - Anti-Src (phospho Tyr529) Antibody (A16498)

Western blot analysis of extracts of A-431 cells, using Anti-Src (phospho Tyr529) Antibody (A16498) at 1:1,000 dilution. A-431 cells were treated by EGF (100 ng/ml) at 37°C for 30 minutes after serum-starvation overnight. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 60s.

Publishing research using Anti-Src (phospho Tyr529) Antibody (A16498)? Please let us know so that we can list the citation on this page.