Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
EBI3-associated protein of 60 kDa, EBIAP, ORCA, OSIL, p60, Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa, Sequestosome-1, Ubiquitin-binding protein p62
Western Blot - Anti-SQSTM1 / p62 Antibody (A80668)
Western blot analysis of extracts of various cell lines, using Anti-SQSTM1 / p62 Antibody (A80668) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.
Western Blot - Anti-SQSTM1 / p62 Antibody (A80668)
Western blot analysis of Jurkat, using Anti-SQSTM1 / p62 Antibody (A80668) at 1:400 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.
Western Blot - Anti-SQSTM1 / p62 Antibody (A80668)
Western blot analysis of extracts from wild type(WT) and SQSTM1/p62 knockout (KO) 293T(KO) cells, using Anti-SQSTM1 / p62 Antibody (A80668) at 1:400 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-SQSTM1 / p62 Antibody (A80668) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunofluorescence analysis of U2OS cells using Anti-SQSTM1 / p62 Antibody (A80668) at a dilution of 1:50 (40x lens). DAPI was used to stain the cell nuclei (blue).
Western Blot - Anti-SQSTM1 / p62 Antibody (A80668)
Immunoprecipitation analysis of 200µg extracts of C6 cells using 3µg of Anti-SQSTM1 / p62 Antibody (A80668). This Western blot was performed on the immunoprecipitate using Anti-SQSTM1 / p62 Antibody (A80668) at a dilution of 1:1000.