Anti-SPAM1 Antibody (A11511)

Name: Anti-SPAM1 Antibody
Brand: Antibodies.com
SKU: A11511
Price: 355.0 USD
Availability: InStock

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$355

Rabbit polyclonal antibody to SPAM1 for WB, IHC and ICC/IF.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-SPAM1 Antibody

Description

Rabbit polyclonal antibody to SPAM1.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 100-300 of human SPAM1 (NP_003108.2).

Sequence

PYIDSITGVTVNGGIPQKISLQDHLDKAKKDITFYMPVDNLGMAVIDWEEWRPTWARNWKPKDVYKNRSIELVQQQNVQLSLTEATEKAKQEFEKAGKDFLVETIKLGKLLRPNHLWGYYLFPDCYNHHYKKPGYNGSCFNVEIKRNDDLSWLWNESTALYPSIYLNTQQSPVAATLYVRNRVREAIRVSKIPDAKSPLPV

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

64 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

Hyal-PH20, HYAL3, Hyaluronidase PH-20, Hyaluronoglucosaminidase PH-20, PH20, Sperm adhesion molecule 1, Sperm surface protein PH-20

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (4)

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Western Blot - Anti-SPAM1 Antibody (A11511)

Western blot analysis of extracts of various cell lines, using Anti-SPAM1 Antibody (A11511) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

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Immunohistochemistry - Anti-SPAM1 Antibody (A11511)

Immunohistochemistry analysis of paraffin-embedded rat testis using Anti-SPAM1 Antibody (A11511) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Immunofluorescence - Anti-SPAM1 Antibody (A11511)

Immunofluorescence analysis of rat testis cells using Anti-SPAM1 Antibody (A11511) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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Immunofluorescence - Anti-SPAM1 Antibody (A11511)

Immunofluorescence analysis of human testis cells using Anti-SPAM1 Antibody (A11511) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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