Anti-SPAK Antibody [ARC1896] (A308775)

Name: Anti-SPAK Antibody [ARC1896]
Brand: Antibodies.com
SKU: A308775
Price: 485.0 USD
Availability: InStock

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$485

Rabbit monoclonal [ARC1896] antibody to SPAK for WB, IHC, ICC/IF and IP.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-SPAK Antibody [ARC1896]

Description

Rabbit monoclonal [ARC1896] antibody to SPAK.

Applications

WB, IHC, ICC/IF, IP

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200, IP: 1:500-1:1,000

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 401-545 of human STK39 (Q9UEW8).

Sequence

SQEKSRRVKEENPEIAVSASTIPEQIQSLSVHDSQGPPNANEDYREASSCAVNLVLRLRNSRKELNDIRFEFTPGRDTADGVSQELFSAGLVDGHDVVIVAANLQKIVDDPKALKTLTFKLASGCDGSEIPDEVKLIGFAQLSVS

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC1896

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

71 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

DCHT, PASK, Serine/threonine-protein kinase 39, Ste-20-related kinase, STE20/SPS1-related proline-alanine-rich protein kinase, STK39

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (6)

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Western Blot - Anti-SPAK Antibody [ARC1896] (A308775)

Western blot analysis of extracts of various cell lines, using Anti-SPAK Antibody [ARC1896] (A308775) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

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Western Blot - Anti-SPAK Antibody [ARC1896] (A308775)

Western blot analysis of extracts of Mouse testis, using Anti-SPAK Antibody [ARC1896] (A308775) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

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Immunohistochemistry - Anti-SPAK Antibody [ARC1896] (A308775)

Immunohistochemistry analysis of paraffin-embedded human colon tissue using Anti-SPAK Antibody [ARC1896] (A308775) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Immunohistochemistry - Anti-SPAK Antibody [ARC1896] (A308775)

Immunohistochemistry analysis of paraffin-embedded human placenta using Anti-SPAK Antibody [ARC1896] (A308775) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.