Anti-SOX10 Antibody (A84577)

Name: Anti-SOX10 Antibody
Brand: Antibodies.com
SKU: A84577
Price: 480.0 USD
Availability: InStock

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$480

Goat polyclonal antibody to SOX10 for ELISA, IF and WB.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 3-5 business days.

Tags:

Neuroepithelial Marker Oligodendrocyte Marker Schwann Cell Marker

Specifications

Name

Anti-SOX10 Antibody

Description

Goat polyclonal antibody to SOX10.

Applications

ELISA, IF, WB

Dilutions

ELISA: 1:128,000, WB: 0.03-0.1µg/ml, IF: 10µg/ml

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide corresponding to the internal region of human SOX10.

Sequence

C-DAKAQVKTETAGPQ

Host

Goat

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 µg/ml

Molecular Weight

50 kDa

Predicted MW

49.9kDa

Product Form

Liquid

Formulation

Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Isotype Controls

Goat IgG (A121671)

Suitable Secondaries

See all Anti-Goat IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (3)

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Western Blot - Anti-SOX10 Antibody (A84577)

SOX10 expression in Human Brain (Cerebellum) lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed for 1 hour with Anti-SOX10 Antibody (A84577) at 0.1µg/ml and detected by chemiluminescence.

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Western Blot - Anti-SOX10 Antibody (A84577)

SOX10 expression in Mouse Brain lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-SOX10 Antibody (A84577) at 0.1µg/ml and detected by chemiluminescence.

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Immunofluorescence - Anti-SOX10 Antibody (A84577)

SOX10 expression in KNRK cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-SOX10 Antibody (A84577) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Nuclear staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.

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