Anti-Sonic Hedgehog Antibody [ARC0701] (A308161)

Name: Anti-Sonic Hedgehog Antibody [ARC0701]
Brand: Antibodies.com
SKU: A308161

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This product is discontinued

Anti-Sonic Hedgehog Antibody [ARC0701] (A308161) has been discontinued and is no longer available.

View all Sonic Hedgehog Antibodies.

Specifications

Name

Anti-Sonic Hedgehog Antibody [ARC0701]

Description

Rabbit monoclonal [ARC0701] antibody to Sonic Hedgehog.

Applications

WB, ICC/IF

Dilutions

WB: 1:500-1:2,000, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 200-300 of human Sonic Hedgehog (Shh) (Q15465).

Sequence

PGSATVHLEQGGTKLVKDLSPGDRVLAADDQGRLLYSDFLTFLDRDDGAKKVFYVIETREPRERLLLTAAHLLFVAPHNDSATGEPEASSGSGPPSGGALG

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC0701

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

50 kDa / 27 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

HHG-1, SHH, Shh unprocessed N-terminal signaling and C-terminal autoprocessing domains, ShhNC, Sonic hedgehog protein

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (3)

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Western Blot - Anti-Sonic Hedgehog Antibody [ARC0701] (A308161)

Western blot analysis of extracts of various cell lines, using Anti-Sonic Hedgehog Antibody [ARC0701] (A308161) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 60s.

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Western Blot - Anti-Sonic Hedgehog Antibody [ARC0701] (A308161)

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Immunofluorescence - Anti-Sonic Hedgehog Antibody [ARC0701] (A308161)

Immunofluorescence analysis of Hep G2 cells using Anti-Sonic Hedgehog Antibody [ARC0701] (A308161) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).