Western blot analysis of extracts of various cell lines, using CCNA2 antibody (A7632) at 1:1,000 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L) (HRP) (AS014) at 1:10,000 dilution. Lysates/proteins: 25µg per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human well-differentiated squamous skin carcinoma using CCNA2 antibody (A7632) at dilution of 1:100 (40x lens).