Rabbit polyclonal antibody to SNAI1 (Phospho-Ser246)
Specificity
The antibody detects endogenous levels of SNAI1 only when phosphorylated at serine 246.
Applications
WB, IF
Dilutions
Western blotting: 1:500~1:1000 Immunofluorescence: 1:100~1:200
Reactivity
Human, Mouse
Immunogen
Peptide sequence around phosphorylation site of Serine 246(T-F-S(p)-R-M) derived from Human SNAI1.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1 mg/ml
Formulation
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage
Store at -20˚C / 1 year
Synonyms
Protein sna, Protein snail homolog 1, SNAH, Zinc finger protein SNAI1
Western blot analysis of extracts from HT29 cells using SNAI1 (Phospho-Ser246) Antibody #11709.The lane on the right is treated with the antigen-specific peptide.